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nls cfp control  (Addgene inc)


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    Addgene inc nls cfp control
    Nls Cfp Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nls+cfp+control/pAd-PV-NLS-CD-DsRed+(Plasmid+%2316345)/pm30416059-257-9-12
    Average 93 stars, based on 3 article reviews
    nls cfp control - by Bioz Stars, 2026-10
    93/100 stars

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    Control:

    Article Title: Callose-Regulated Symplastic Communication Coordinates Symbiotic Root Nodule Development.
    Article Snippet: Critical Commercial Assays Gateway BP and LR clonase II enzyme mixes Invitrogen Cat#11789-100 and Cat#11791-100, respectively Bsa I enzyme for Goldengate reactions New England BIOLABS Cat#R0535S X-Gluc (5-bromo-4-chloro-3-indoxyl-b-D-GlcA, cyclohexylammonium salt Biosynth Cat#B7300; CAS: 114162-64-0 Magenta-Gal substrate (5-bromo-6-chloro-3-indoxyl-b-Dgalactopyranoside Biosynth Cat#B7200; CAS: 93863-88-8 X-Gal substrate (5-bromo-4-chloro-3-indolyl-b-Dgalactopyranoside Fisher BioReagents Cat#10113253; CAS: 7240-90-6 Technovit 7100 Heraeus-Kulzer, Germany Cat#64709003 Macherey-Nagel NUCLEOSPIN RNA kit Macherey-Nagel Cat#740955.250 Agilent RNA Nano Chip and Reagents Agilent Technologies Cat#5067-1511 Transcriptor Reverse Transcriptase for QRT-PCR Roche Cat#03531287001 LightCycler480 Sybr Green I Master Roche Cat#04707516001 Anilin Blue EMS Cat#10840; CAS: 28631-66-5 glutaraldehyde 25% EMS Cat#16210; CAS: 111-30-8 Paraformaldehyde aqueous solution 32% EMS Cat#15714; CAS: 30525-89-4 5(6)-Carboxyfluorescein diacetate (CFDA) Sigma-Aldrich Cat#21879; CAS: 124387-19-5 Ruthenium red Sigma-Aldrich Cat#R-2751; CAS: 11103-72-3 Recombinant DNA PdBG1(At3g13560)-eGFP protein fusion GenScript, this work PdBG1-GFP MtBG2 (Medtr3g083580)-mCherry protein fusion GenScript, this work MtBG2-mCherry pMtBG2:GUS in a pCambiaCR1 derived vector This work pMtBG2:GUS 1.4 kb pNIN:GUS reporter in a pCambiaCR1 derived vector This work 1.4 kb pNIN:GUS 2.1 kb pNIN:GUS reporter in a pCambiaCR1 derived vector This work 2.1 kb pNIN:GUS 2.2 kb pERN1:GUS reporter in a pCambiaCR1 derived vector This work pERN1:GUS 2.2Kb pERN1-MtBG2 RNAi-50UTR in a pCambiaCR1 derived vector This work MtBG2 RNAi-50 2.2Kb pERN1-MtBG2 RNAi-cds(850bp) in a pCambiaCR1 derived vector This work MtBG2 RNAi-cds 2.2 Kb pNFYA1:GUS in a pK7m34GW vector [42] pNF-YA1:GUS pEXPA:YFP in a pCambiaCR1 derived vector [30] and this work pEXPA:YFP pEXPA:mCherry-ER in a pCambiaCR1 derived vector [30] and this work pEXPA:mCherry-ER pEXPA:cals3m in a pK7WG2-R derived vector This work pEXPA:cals3m (Continued on next page) Current Biology 28, 1–16.e1–e6, November 19, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER pEXPA:PVcd (PVcd, parvalbumin mutated) :NLS:CFP control [54] Addgene#16345, modified in this work pERN1:cals3m in a pK7WG2-R derived vector This work pERN1:cals3m pERN1:GFP in a pK7WG2-R derived vector This work pERN1:GFP pMtBG2:cals3m in a pK7WG2-R derived vector This work pMtBG2:cals3m pMtBG2:GFP in a pK7WG2-R derived vector This work pMtBG2:GFP pANN1:cals3m in a pK7WG2 derived vector This work pANN1:cals3m pANN1:GFP in a pK7WG2 derived vector This work pANN1:GFP pDONR221-cals3m [37], kindly provided by Yk€a Helariutta pDONR221-cals3m pCambiaCR1 vectors [55] N/A pK7WG2-R-pLjUb vector [56] N/A Oligonucleotides See Table S2 for the List of primers used in this study. .. This work N/A Deposited Data Raw and analyzed data This work Medeley database; https://doi.org/ 10.17632/5hzcxdb4dp.1 Software and Algorithms Leica, ImageJ [57] http://imagej.nih.gov/ij/ Volocity version 6.0.1 software PerkinElmer License n 147.99.102.74 Figtree http://tree.bio.ed.ac.uk/ software/figtree/ N/A TreeGraph2 [58] http://treegraph.bioinfweb.info/ R software http://r-project.org N/A

    Modification:

    Article Title: Callose-Regulated Symplastic Communication Coordinates Symbiotic Root Nodule Development.
    Article Snippet: Critical Commercial Assays Gateway BP and LR clonase II enzyme mixes Invitrogen Cat#11789-100 and Cat#11791-100, respectively Bsa I enzyme for Goldengate reactions New England BIOLABS Cat#R0535S X-Gluc (5-bromo-4-chloro-3-indoxyl-b-D-GlcA, cyclohexylammonium salt Biosynth Cat#B7300; CAS: 114162-64-0 Magenta-Gal substrate (5-bromo-6-chloro-3-indoxyl-b-Dgalactopyranoside Biosynth Cat#B7200; CAS: 93863-88-8 X-Gal substrate (5-bromo-4-chloro-3-indolyl-b-Dgalactopyranoside Fisher BioReagents Cat#10113253; CAS: 7240-90-6 Technovit 7100 Heraeus-Kulzer, Germany Cat#64709003 Macherey-Nagel NUCLEOSPIN RNA kit Macherey-Nagel Cat#740955.250 Agilent RNA Nano Chip and Reagents Agilent Technologies Cat#5067-1511 Transcriptor Reverse Transcriptase for QRT-PCR Roche Cat#03531287001 LightCycler480 Sybr Green I Master Roche Cat#04707516001 Anilin Blue EMS Cat#10840; CAS: 28631-66-5 glutaraldehyde 25% EMS Cat#16210; CAS: 111-30-8 Paraformaldehyde aqueous solution 32% EMS Cat#15714; CAS: 30525-89-4 5(6)-Carboxyfluorescein diacetate (CFDA) Sigma-Aldrich Cat#21879; CAS: 124387-19-5 Ruthenium red Sigma-Aldrich Cat#R-2751; CAS: 11103-72-3 Recombinant DNA PdBG1(At3g13560)-eGFP protein fusion GenScript, this work PdBG1-GFP MtBG2 (Medtr3g083580)-mCherry protein fusion GenScript, this work MtBG2-mCherry pMtBG2:GUS in a pCambiaCR1 derived vector This work pMtBG2:GUS 1.4 kb pNIN:GUS reporter in a pCambiaCR1 derived vector This work 1.4 kb pNIN:GUS 2.1 kb pNIN:GUS reporter in a pCambiaCR1 derived vector This work 2.1 kb pNIN:GUS 2.2 kb pERN1:GUS reporter in a pCambiaCR1 derived vector This work pERN1:GUS 2.2Kb pERN1-MtBG2 RNAi-50UTR in a pCambiaCR1 derived vector This work MtBG2 RNAi-50 2.2Kb pERN1-MtBG2 RNAi-cds(850bp) in a pCambiaCR1 derived vector This work MtBG2 RNAi-cds 2.2 Kb pNFYA1:GUS in a pK7m34GW vector [42] pNF-YA1:GUS pEXPA:YFP in a pCambiaCR1 derived vector [30] and this work pEXPA:YFP pEXPA:mCherry-ER in a pCambiaCR1 derived vector [30] and this work pEXPA:mCherry-ER pEXPA:cals3m in a pK7WG2-R derived vector This work pEXPA:cals3m (Continued on next page) Current Biology 28, 1–16.e1–e6, November 19, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER pEXPA:PVcd (PVcd, parvalbumin mutated) :NLS:CFP control [54] Addgene#16345, modified in this work pERN1:cals3m in a pK7WG2-R derived vector This work pERN1:cals3m pERN1:GFP in a pK7WG2-R derived vector This work pERN1:GFP pMtBG2:cals3m in a pK7WG2-R derived vector This work pMtBG2:cals3m pMtBG2:GFP in a pK7WG2-R derived vector This work pMtBG2:GFP pANN1:cals3m in a pK7WG2 derived vector This work pANN1:cals3m pANN1:GFP in a pK7WG2 derived vector This work pANN1:GFP pDONR221-cals3m [37], kindly provided by Yk€a Helariutta pDONR221-cals3m pCambiaCR1 vectors [55] N/A pK7WG2-R-pLjUb vector [56] N/A Oligonucleotides See Table S2 for the List of primers used in this study. .. This work N/A Deposited Data Raw and analyzed data This work Medeley database; https://doi.org/ 10.17632/5hzcxdb4dp.1 Software and Algorithms Leica, ImageJ [57] http://imagej.nih.gov/ij/ Volocity version 6.0.1 software PerkinElmer License n 147.99.102.74 Figtree http://tree.bio.ed.ac.uk/ software/figtree/ N/A TreeGraph2 [58] http://treegraph.bioinfweb.info/ R software http://r-project.org N/A

    Derivative Assay:

    Article Title: Callose-Regulated Symplastic Communication Coordinates Symbiotic Root Nodule Development.
    Article Snippet: Critical Commercial Assays Gateway BP and LR clonase II enzyme mixes Invitrogen Cat#11789-100 and Cat#11791-100, respectively Bsa I enzyme for Goldengate reactions New England BIOLABS Cat#R0535S X-Gluc (5-bromo-4-chloro-3-indoxyl-b-D-GlcA, cyclohexylammonium salt Biosynth Cat#B7300; CAS: 114162-64-0 Magenta-Gal substrate (5-bromo-6-chloro-3-indoxyl-b-Dgalactopyranoside Biosynth Cat#B7200; CAS: 93863-88-8 X-Gal substrate (5-bromo-4-chloro-3-indolyl-b-Dgalactopyranoside Fisher BioReagents Cat#10113253; CAS: 7240-90-6 Technovit 7100 Heraeus-Kulzer, Germany Cat#64709003 Macherey-Nagel NUCLEOSPIN RNA kit Macherey-Nagel Cat#740955.250 Agilent RNA Nano Chip and Reagents Agilent Technologies Cat#5067-1511 Transcriptor Reverse Transcriptase for QRT-PCR Roche Cat#03531287001 LightCycler480 Sybr Green I Master Roche Cat#04707516001 Anilin Blue EMS Cat#10840; CAS: 28631-66-5 glutaraldehyde 25% EMS Cat#16210; CAS: 111-30-8 Paraformaldehyde aqueous solution 32% EMS Cat#15714; CAS: 30525-89-4 5(6)-Carboxyfluorescein diacetate (CFDA) Sigma-Aldrich Cat#21879; CAS: 124387-19-5 Ruthenium red Sigma-Aldrich Cat#R-2751; CAS: 11103-72-3 Recombinant DNA PdBG1(At3g13560)-eGFP protein fusion GenScript, this work PdBG1-GFP MtBG2 (Medtr3g083580)-mCherry protein fusion GenScript, this work MtBG2-mCherry pMtBG2:GUS in a pCambiaCR1 derived vector This work pMtBG2:GUS 1.4 kb pNIN:GUS reporter in a pCambiaCR1 derived vector This work 1.4 kb pNIN:GUS 2.1 kb pNIN:GUS reporter in a pCambiaCR1 derived vector This work 2.1 kb pNIN:GUS 2.2 kb pERN1:GUS reporter in a pCambiaCR1 derived vector This work pERN1:GUS 2.2Kb pERN1-MtBG2 RNAi-50UTR in a pCambiaCR1 derived vector This work MtBG2 RNAi-50 2.2Kb pERN1-MtBG2 RNAi-cds(850bp) in a pCambiaCR1 derived vector This work MtBG2 RNAi-cds 2.2 Kb pNFYA1:GUS in a pK7m34GW vector [42] pNF-YA1:GUS pEXPA:YFP in a pCambiaCR1 derived vector [30] and this work pEXPA:YFP pEXPA:mCherry-ER in a pCambiaCR1 derived vector [30] and this work pEXPA:mCherry-ER pEXPA:cals3m in a pK7WG2-R derived vector This work pEXPA:cals3m (Continued on next page) Current Biology 28, 1–16.e1–e6, November 19, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER pEXPA:PVcd (PVcd, parvalbumin mutated) :NLS:CFP control [54] Addgene#16345, modified in this work pERN1:cals3m in a pK7WG2-R derived vector This work pERN1:cals3m pERN1:GFP in a pK7WG2-R derived vector This work pERN1:GFP pMtBG2:cals3m in a pK7WG2-R derived vector This work pMtBG2:cals3m pMtBG2:GFP in a pK7WG2-R derived vector This work pMtBG2:GFP pANN1:cals3m in a pK7WG2 derived vector This work pANN1:cals3m pANN1:GFP in a pK7WG2 derived vector This work pANN1:GFP pDONR221-cals3m [37], kindly provided by Yk€a Helariutta pDONR221-cals3m pCambiaCR1 vectors [55] N/A pK7WG2-R-pLjUb vector [56] N/A Oligonucleotides See Table S2 for the List of primers used in this study. .. This work N/A Deposited Data Raw and analyzed data This work Medeley database; https://doi.org/ 10.17632/5hzcxdb4dp.1 Software and Algorithms Leica, ImageJ [57] http://imagej.nih.gov/ij/ Volocity version 6.0.1 software PerkinElmer License n 147.99.102.74 Figtree http://tree.bio.ed.ac.uk/ software/figtree/ N/A TreeGraph2 [58] http://treegraph.bioinfweb.info/ R software http://r-project.org N/A



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